Next-Generation Sequencing Data Analysis

NEXT – GENERATION SEQUENCING DATA ANALYSIS

NEXT – GENERATION SEQUENCING DATA ANALYSIS program covers following topics:
  • Biological Database like NCBI, EMBL, PDB, Uniprot, DDBJ, KEGG etc.
  • Sequence Alignment employing BLAST, FASTA, CLUSTAL
  • Phylogenetic Relationship using MEGA, CLUSTAL and Phylip
  • Capillary Electrophoresis
  • Analysis of DNA Purity
  • Introduction to Illumina Sequencing
  • Illumina Sequencing Tools
  • Denovo genome sequencing
  • Genome Re-sequencing and Variant calling
  • Genotyping by Sequencing
  • 16s Metagenome sequencing
  • Fungal ITS sequencing
  • Shotgun Metagenome sequencing
  • Denovo transcriptome sequencing
  • Reference based mRNA/transcriptome sequencing
  • Meta transcriptome sequencing
  • ChIP sequencing
  • miRNA sequencing
  • RIP sequencing
  • ATAC sequencing
  • Methylome sequencing
  • degradome sequencing
  • Advance NGS Tools like Fastqc , Prokka etc
  • Gene Prediction and Introduction to sequencing methods (Illumina Sequencing)
  • Pipelines workflow like metagenome analysis
  • NGS Data Visualization using fastq and fastqc