Small RNA sequencing has become a powerful tool for understanding gene regulation, disease mechanisms, and RNA-based therapeutics. Accurate identification of miRNA and siRNA from NGS data requires a robust and scientifically validated bioinformatics workflow. At BioNome, we provide end-to-end small RNA sequencing analysis services tailored to researchers, pharmaceutical companies, and biotech startups across India.
Recognized as the Best Bioinformatics Service Provider in Bangalore (Karnataka), BioNome delivers affordable bioinformatics services with high analytical accuracy and comprehensive reporting.
Step 1: Data Quality Control & Preprocessing
The workflow begins with rigorous quality assessment of raw FASTQ files. This includes:
Quality score evaluation
Adapter trimming (critical for small RNA data)
Removal of low-quality reads
Filtering of contaminants and artifacts
Accurate preprocessing ensures reliable downstream analysis.
Step 2: Alignment to Reference Genome
Cleaned reads are mapped to the appropriate reference genome using optimized alignment tools. This step enables:
Identification of known miRNAs using reference databases
Detection of siRNA sequences
Filtering of non-specific matches
Precise alignment minimizes false positives and improves confidence in results.
Step 3: Novel miRNA & siRNA Discovery
Beyond known sequences, BioNome performs computational prediction of novel small RNAs by:
Identifying characteristic hairpin structures
Analyzing read distribution patterns
Evaluating thermodynamic stability
Confirming structural features of precursor miRNAs
This step expands the discovery potential for new regulatory RNAs.
Step 4: Differential Expression Analysis
We conduct statistical analysis to identify significantly upregulated or downregulated miRNAs/siRNAs between experimental groups. This supports:
Biomarker discovery
Disease association studies
Functional genomics research
RNA-based therapeutic development
Step 5: Target Prediction & Functional Annotation
Identified miRNAs and siRNAs are further analyzed for:
Target gene prediction
Gene Ontology (GO) enrichment
Pathway analysis (KEGG and related databases)
Regulatory network construction
This provides biological context and actionable insights.
Why Choose BioNome?
BioNome combines advanced computational tools, experienced bioinformaticians, and scalable infrastructure to deliver accurate NGS analysis. Our affordable bioinformatics services support transcriptomics, RNA-Seq, small RNA sequencing, and AI-driven genomics research.
Contact BioNome
📞 Phone: +91 8668470445
📧 Email: info@bionome.in
Partner with BioNome in India for reliable miRNA & siRNA identification from NGS data, ensuring precise analysis and meaningful biological insights for your research projects.